Protein Crystallography

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis.

Author: Alexander Wlodawer

Publisher: Humana

ISBN: 9781493983636

Category: Science

Page: 672

View: 286

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.

Protein Crystallography

In some fibers, such as F-actin, the repeating unit is an entire protein molecule. A
macroscopic fiber is composed of many of these units, with their long axes
approximately parallel. However, these units are randomly oriented about their
long ...

Author: Eaton E. Lattman

Publisher: JHU Press

ISBN: 0801888069

Category: Science

Page: 152

View: 772

The proteome remains a mysterious realm. Researchers have determined the structures of only a small fraction of the proteins encoded by the human genome. Crystallography continues to be the primary method used to determine the structures of the remaining unknown proteins. This imaging technique uses the diffraction of X-rays to determine a protein's three-dimensional molecular structure. Drawing on years of research and teaching experience, Eaton E. Lattman and Patrick J. Loll use clear examples and abundant illustrations to provide a concise and accessible primer on protein crystallography. Discussing the basics of diffraction, the behavior of two- and three-dimensional crystals, phase determination (including MIR and MAD phasing and molecular replacement), the Patterson function, and refinement, Lattman and Loll provide a complete overview of this important technique, illuminated by physical insights. The crisp writing style and simple illustrations will provide beginner crystallographers with a guide to the process of unraveling protein structure.

Protein Crystallography in Drug Discovery

Harren Jhoti 10.1 Introduction In the late 1990s the concept of high-throughput
crystallography was first proposed. ... Furthermore, the ability to rapidly obtain
crystal structures of a target protein in complex with small molecules is driving a
new ...

Author: Robert E. Babine

Publisher: John Wiley & Sons

ISBN: 3527606351

Category: Science

Page: 278

View: 520

The rational, structure-based approach has become standard in present-day drug design. As a consequence, the availability of high-resolution structures of target proteins is more often than not the basis for an entire drug development program. Protein structures suited for rational drug design are almost exclusively derived from crystallographic studies, and drug developers are relying heavily on the power of this method. Here, researchers from leading pharmaceutical companies present valuable first-hand information, much of it published for the first time. They discuss strategies to derive high-resolution structures for such important target protein classes as kinases or proteases, as well as selected examples of successful protein crystallographic studies. A special section on recent methodological developments, such as for high-throughput crystallography and microcrystallization, is also included. A valuable companion for crystallographers involved in protein structure determination as well as drug developers pursuing the structure-based approach for use in their daily work.

Protein Crystallography

Then in 2011 a new chapter opened with the first publications of results from a
hard X-ray free electron laser (XFEL), the ultimate impact of XFEL on protein
crystallography is still an open question, and is discussed in the current volume.

Author: Konstantinos Beis

Publisher: Royal Society of Chemistry

ISBN: 1788014774

Category: Science

Page: 228

View: 141

Protein crystallography has become vital to further understanding the structure and function of many complex biological systems. In recent years, structure determination has progressed tremendously however the quality of crystals and data sets can prevent the best results from being obtained. With contributions from world leading researchers whose software are used worldwide, this book provides a coherent approach on how to handle difficult crystallographic data and how to assess its quality. The chapters will cover all key aspects of protein crystallography, from instrumentation and data processing through to model building. This book also addresses challenges that protein crystallographers will face such as dealing with data from microcrystals and multi protein complexes. This book is ideal for both academics and researchers in industry looking for a comprehensive guide to protein crystallography.

Practical Protein Crystallography

This book is a practical handbook intended to be used by anyone who wants to
solve a structure by protein crystallography. It should prove useful both to new
protein crystallographers and to old hands. While users of this handbook should
be ...

Author: Duncan E. McRee

Publisher: Academic Press

ISBN: 0323138888

Category: Science

Page: 386

View: 704

Designed for easy use by both new and experienced protein crystallographers, this much-needed book is for anyone interested in solving protein structures by the method of crystallography. It contains many examples ofactual experiments and data, including electron density maps. Computer methods and computer code samples are presented. Practical Protein Crystallography is loaded with new information on area detectors, synchrotron radiation techniques, and the latest computer methods, and features the XtalView software system. Graduate students and teachers in physical biochemistry and pharmaceutical researchers will find this text a timely and convenient aid.

Principles of Protein X ray Crystallography

An optimal choice for protein crystallography is the Cu Kct wavelength of 1.5418
A. However, if high intensity synchrotron radiation is available, a shorter
wavelength, e.g., near 1A, has the advantage of lower absorption (Gonzalez,
1994; ...

Author: Jan Drenth

Publisher: Springer Science & Business Media

ISBN: 9780387985879

Category: Science

Page: 341

View: 766

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive intro ductions to their fields. These should serve the needs of one-semester or one-quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses. Charles R. Cantor v Preface to the Second Edition Since the publication of the previous edition in 1994, X-ray crystallography of proteins has advanced by improvements in existing techniques and by addition of new techniques.

Structure Determination by X Ray Crystallography

Most proteins rapidly deteriorate once exposed to x-rays, through the formation of
free radicals. Crystal lifetime can ... These two factors are highly desirable in
protein crystallography, as protein crystals are notoriously poor diffractors. Since
the ...

Author: Mark F.C. Ladd

Publisher: Springer Science & Business Media

ISBN: 9780306474545

Category: Science

Page: 819

View: 472

This book is an account of crystal symmetry and optical and e-ray diffraction techniques for examining single crystals. It includes the solution of crystal structures by the current methods, worked examples of crystal structure determination, problems and solutions for each chapter.

Protein Crystallography

Several new techniques have been specially developed for protein
crystallography . These are considered in Chapter 15 . Analysis of the results of
the refinements by these methods indicate that very much more detailed
information on protein ...

Author: T. L. Blundell

Publisher: Academic Press

ISBN:

Category: Science

Page: 565

View: 867


A NASA Recipe for Protein Crystallography

Includes an experiment showing how to make protein crystals from Brazil nuts.

Author:

Publisher:

ISBN:

Category: Proteins

Page:

View: 177

Includes an experiment showing how to make protein crystals from Brazil nuts.

Crystallography Made Crystal Clear

An Overview of Protein Crystallography I. Introduction The most common means
of obtaining a detailed picture of a large molecule, allowing the resolution of
individual atoms, is to interpret the diffraction of x-rays from many identical
molecules ...

Author: Gale Rhodes

Publisher: Academic Press

ISBN: 0323137784

Category: Science

Page: 202

View: 417

Crystallography Made Crystal Clear is designed to meet the need for an X-ray analysis that is between brief textbook sections and complete treatments. The book provides non-crystallographers with an intellectually satisfying explanation of the principles of how protein models are gleaned from X-ray analysis. The understanding of these concepts will foster wise use of the models, including the recognition of the strengths and weaknesses of pictures or computer graphics. Since proteins comprise the majority of the mass of macromolecules in cells and carry out biologically important tasks, the book will be of interest to biologists. Provides accessible descriptions of principles of x-ray crystallography, built on simple foundations for anyone with a basic science background Leads the reader through clear, thorough, unintimidating explanations of the mathematics behind crystallography Explains how to read crystallography papers in research journals If you use computer-generated models of proteins or nucleic acids for: Studying molecular interactions Designing ligands, inhibitors, or drugs Engineering new protein functions Interpreting chemical, kinetic, thermodynamic, or spectroscopic data Studying protein folding Teaching macromolecule structure,and if you want to read new structure papers intelligently; become a wiser user of macromolecular models; and want to introduce undergraduates to the important subject of x-ray crystallography, then this book is for you.

Low Temperature Protein Crystallography

Protein Crystallography , Academic Press , London . 37 . Wyckoff , H. W. , Do
scher , M. , Tsernoglow , D. , Inagami , T. , Johnson , L. N. , Hardman , K. D. ,
Allewell , N. M. , Kelly , D. M. , and Richards , F. M. ( 1967 ) . J. Mol . Biol . 27 , 563
. 38 .

Author: Carl William Cork

Publisher:

ISBN:

Category: Elastases

Page: 322

View: 242


Fundamentals of Crystallography

9.1 Introduction Protein crystallography, the subject of this chapter, is a
specialized branch of crystallography that ... 1 " Most of them, like proteins,
transfer-ribonucleic acid (t-RNA). polysaccharides, polynucleotides (and
complexes of all of the ...

Author: Carmelo Giacovazzo

Publisher: Oxford University Press, USA

ISBN: 9780198509585

Category: Science

Page: 825

View: 939

Crystallography and structure theory have recently received increasing interest due to their role in understanding biological structures, high-temperature superconductors, and effects on mineral properties related to changes in temperature and pressure. This text offers an account of the wide range of crystallography in various branches of science.The fundamentals, the most frequently used procedures, and experimental techniques are all described, and a number of appendices are devoted to more specialist aspects. The book is an updated edition with emphasis on the range of topical applications. Illustrations should help to clarify the subject matter. To provide a better understanding of the basics of crystallography, a compact disk has been added to this edition, offering the facilities of modern graphics to simulate experiments, show complex images, and provide a number of exercises.

Biomolecular Crystallography

... published their classic text Protein Crystallography. For many years it was
regarded as the definitive reference for practitioners of X-ray structure analysis.
Bernhard Rupp's Biomolecular Crystallography is, in every way, a worthy
successor.

Author: Bernhard Rupp

Publisher: Garland Science

ISBN: 1134064195

Category: Science

Page: 800

View: 884

Synthesizing over thirty years of advances into a comprehensive textbook, Biomolecular Crystallography describes the fundamentals, practices, and applications of protein crystallography. Deftly illustrated in full-color by the author, the text describes mathematical and physical concepts in accessible and accurate language. It distills key co

Structural Electron Crystallography

Crystallization from Solution The study of three-dimensional protein crystals by
electron crystallographic methods is often motivated by frustrated attempts to
obtain single crystals of suitable size for data collection on an x-ray diffractometer
.

Author: D.L. Dorset

Publisher: Springer Science & Business Media

ISBN: 9780306450495

Category: Science

Page: 452

View: 193

This authoritative text on electron diffraction and crystal structure analysis is the first to describe direct phasing techniques in electron crystallography. Written for electron diffractionists and electron microscopists, this fully illustrated volume presents methods for specimen preparation, data collection and structure analysis. Chapters feature numerous detailed examples of actual structure analyses and contain over 350 illustrations.

Protein Structure

In this case, the crystal is assembled from the known molecular models so that
the calculated diffraction pattern fits the observed ... The MR methods can thus be
expected to play a central role in this new era of protein crystallography, when
the ...

Author: Daniel Chasman

Publisher: CRC Press

ISBN: 0824748166

Category: Medical

Page: 576

View: 728

This text offers in-depth perspectives on every aspect of protein structure identification, assessment, characterization, and utilization, for a clear understanding of the diversity of protein shapes, variations in protein function, and structure-based drug design. The authors cover numerous high-throughput technologies as well as computational methods to study protein structures and residues. A valuable reference, this book reflects current trends in the effort to solve new structures arising from genome initiatives, details methods to detect and identify errors in the prediction of protein structural models, and outlines challenges in the conversion of routine processes into high-throughput platforms.

Macromolecular Crystallography with Synchrotron Radiation

CHAPTER 10 More applications 10.1 EARLY HISTORY AND GENERAL
INTRODUCTION The first discussions in the literature concerning the
applications of SR in protein crystallography were given by Harrison (1973),
Wyckoff (1973) and ...

Author: John R. Helliwell

Publisher: Cambridge University Press

ISBN: 9780521544047

Category: Science

Page: 595

View: 916

This highly illustrated monograph provides a comprehensive study of the structure and function of proteins, nucleic acids and viruses using synchrotron radiation and crystallography. Synchrotron radiation is intense, polychromatic and finely collimated, and is highly effective for probing the structure of macromolecules. This is a fast-expanding field, and this timely monograph gives a complete introduction to the technique and its uses. Beginning with chapters on the fundamentals of macromolecular crystallography and macromolecular structure, the book goes on to review the sources and properties of synchrotron radiation, instrumentation and data collection. There are chapters on the Laue method, on diffuse X-ray scattering and on variable wavelength dispersion methods. The book concludes with a description and survey of applications including studies at high resolution, the use of small crystals, the study of large unit cells, and time-resolved crystallography (particularly of enzymes). Appendices are provided which present essential information for the synchrotron user as well as information about synchrotron facilities currently available. A detailed bibliography and reference section completes the volume. Many tables, diagrams and photographs are included.

Macromolecular Crystallography Protocols

Introduction Taking advantage of the structural similarity of proteins with high-
sequence homology is a conceptually straightforward way to overcome the
phase problem in protein crystallography (1). Unfortunately, this method, known
as ...

Author: Sylvie Doublié

Publisher: Springer Science & Business Media

ISBN: 1597452661

Category: Science

Page: 323

View: 679

In the decade since publication of the first edition this book, the field has seen several major developments. These developments have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I is dedicated to crystallization and ways to increase the odds of obtaining crystals in macromolecules. Volume 2 covers both computational methods for characterizing crystals and solving structures.

Time resolved Macromolecular Crystallography

Can Laue catch Maxwell ?: observation of shortlived species by Laue X - ray
crystallography BY DAGMAR RINGE ... North Carolina , U.S.A. Now that the Laue
method has been established as a tool for protein crystallography , the main ...

Author: Royal Society (Great Britain)

Publisher: Oxford [England] ; New York : Oxford University Press

ISBN: 9780198557814

Category: Science

Page: 334

View: 758

X-ray diffraction studies have made outstanding contributions to structural molecular biology. The advent of high-intensity synchrotron radiation sources, coupled with the revival of the Laue method, has now made possible the rapid collection of X-ray crystallography data. As a result, proteinand virus crystallography is now progressing from studies of equilibrium structures to time-resolved studies of structures at reaction stages. The full exploitation of the synchrotron Laue method for the study of dynamic events in crystals requires many developments in physics, chemistry, andbiochemistry: the papers presented in this volume aim to report some of the striking results that have already been obtained and to discuss and identify necessary future developments.

Macromolecular Crystallography Part D

In general , in this approach the initial phases are derived from differences in
crystal scattering caused by the presence of a small number of heavy ... An
example of the former is 1 T. L. Blundell and L. N. Johnson , “ Protein
Crystallography .

Author: Charles W. Carter, Jr.

Publisher: Gulf Professional Publishing

ISBN: 9780121827779

Category: Science

Page: 679

View: 692

Accurate molecular structures is vital for rational drug design and for structure based functional studies directed toward the development of effective therapeutic agents and drugs. Crystallography can reliably predict structure, both in terms of folding and atomic details of bonding. * Phases * Map interpretation and refinement * Analysis and software

Neutron Scattering in Biology

It is in these more mobile parts of the protein structure that the role of neutron
protein crystallography in completing the protein structure protonation details is
clearly needed . It is also a fair question to ask just how many protein crystal ...

Author: Jörg Fitter

Publisher: Springer Science & Business Media

ISBN: 9783540291084

Category: Medical

Page: 557

View: 185

The advent of new neutron facilities and the improvement of existing sources and instruments world wide supply the biological community with many new opportunities in the areas of structural biology and biological physics. The present volume offers a clear description of the various neutron-scattering techniques currently being used to answer biologically relevant questions. Their utility is illustrated through examples by some of the leading researchers in the field of neutron scattering. This volume will be a reference for researchers and a step-by-step guide for young scientists entering the field and the advanced graduate student.