Yeast Protocols

8 Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes Laura
Maringele and David Lydall Summary Pulsed-field gel electrophoresis (PFGE)
can be used to separate the 16 budding yeast chromosomes on the basis of size.

Author: Wei Xiao

Publisher: Springer Science & Business Media

ISBN: 1592599583

Category: Science

Page: 392

View: 906

Since the publication of Yeast Protocols in 1996, many new techniques have been invented and original protocols improved and refined. This throughly updated second edition will serve as a stand-alone protocols handbook of these new and refined techniques suitable for daily use in all research laboratories. It includes all of the recent advanced protocols as well as the major basic techniques and hence, will be essential for both yeast research laboratories and those researchers who wish to use yeast as a host to study their favorite genes from other organisms.

Yeast Sugar Metabolism

Advances in research on metabolite transport into yeasts stemmed from the
pioneering work on bacteria . In the 1950s Monod described saturation kinetics
and active transport in Escherichia coli , and in the 1960s Mitchell was
responsible for ...

Author: Friedrich K. Zimmermann

Publisher: CRC Press

ISBN: 9781566764667

Category: Technology & Engineering

Page: 567

View: 648

Guide to Yeast Genetics and Molecular Biology

[1] Getting Started with Yeast By Fred Sherman Virtues of Yeast The yeast
Saccharomyces cerevisiae is recognized as an ideal eukaryotic microorganism
for biological studies. Although yeasts have greater genetic complexity than
bacteria, ...

Author: Christine Guthrie

Publisher: Gulf Professional Publishing

ISBN: 9780121827786

Category: Science

Page: 933

View: 881

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein

Use of Yeast Biomass in Food Production

The addition of yeast extract also slightly increased the protein content of yeasts .
Mahmoud and Kosikowski169 stated that supplementation of peptone resulted in
the highest biomass yield with minimum alcohol production . This result is in ...

Author: Anna Halasz

Publisher: CRC Press

ISBN: 9780849358661

Category: Technology & Engineering

Page: 352

View: 100

Yeast biomass is an excellent source of proteins, nucleic acids, and vitamins. It has been produced and consumed in baked goods and other foods for thousands of years and offers significant advantages when compared to other potential new microbial protein sources. Use of Yeast Biomass in Food Production provides up-to-date information regarding the chemical composition and biochemistry of yeasts, discusses the biotechnological basis of yeast production and possibilities for influencing yeast biomass composition using new techniques in molecular biology. The book examines techniques for producing yeast protein concentrates (and isolates) while still retaining their functional properties and nutritive values, as well as the various uses for these materials and their derivatives in different branches of the food industry. Finally, the book explores possibilities for the production and industrial use of other yeast components, such as nucleic acids, nucleotides, cell wall polysaccharides, autolysates, and extracts. Food microbiologists and technologists, as well as biotechnologists, will discover that this book is an invaluable reference resource.

Yeast Strain Selection

cr Wine Yeast : Selection and Modification Ronald Ernest Subden University of
Guelph Guelph , Ontario , Canada 1. INTRODUCTION A. Historical This chapter
provides a historical perspective on the use of pure starter culture yeasts for wine

Author: Panchal

Publisher: CRC Press

ISBN: 9780824782764

Category: Technology & Engineering

Page: 368

View: 719

Contributors from universities and food, pharmaceutical, and brewing companies detail the current state of yeast strain development and handling, highlighting advances in yeast selection for academic research, industry, and recombinant DNA technology. Featuring the use of Saccharomyces and other yea

The Yeasts

The sixth and final volume of the exceptional Yeasts Series this book is a fully edited, research-level treatise on yeast genetics.

Author: Anthony H. Rose

Publisher: Academic Press


Category: Science

Page: 660

View: 459

Yeasts are highly versatile organisms, particularly suitable for industrial purposes. This book covers the major areas of yeast technology relevant to the food, pharmaceutical and biotechnology industries.

Yeast Physiology and Biotechnology

The diverse and dynamic activities of yeasts impinge on many areas of science ,
technology and medicine . Some species play beneficial roles in the production
of foods , beverages and pharmaceuticals , while others play detrimental roles as

Author: Graeme M. Walker

Publisher: John Wiley & Sons

ISBN: 9780471964469

Category: Science

Page: 362

View: 114

Yeasts are the world's premier industrial micro-organisms. In addition to their wide exploitation in the production of foods, beverages and pharmaceuticals, yeasts also play significant roles as model eukaryotic cells in furthering our knowledge in the biological and biomedical sciences. In order for modern biotechnology to fully exploit the activities of yeasts, it is essential to appreciate aspects of yeast cell physiology. In recent years, however, our knowledge of yeast physiological phenomena has lagged behind that of yeast genetics and molecular biology. Yeast Physiology and Biotechnology redresses the balance by linking key aspects of yeast physiology with yeast biotechnology. Individual chapters provide broad and timely coverage of yeast cytology, nutrition, growth and metabolism - important aspects of yeast cell physiology which are pertinent to the practical uses of yeasts in industry. The final chapter reviews traditional, modern and emerging biotechnologies in which roles of yeasts in the production of industrial commodities and their value in biomedical research are fully discussed. Relevant aspects of classical and modern yeast genetics and molecular biology are fully integrated into the appropriate chapters. This up-to-date and fully referenced book is aimed at advanced undergraduate and postgraduate bioscience students,but will also prove to be a valuable source of information for yeast researchers and technologists.

Yeast Cells

Growth of Yeasts A. Composition of Growth Media 1. General Growth Media The
recommended general media for growing yeasts (van der Walt, 1970) are malt
extract and Wickerham's (1951) MYGP medium. Either medium, liquid or
solidified, ...


Publisher: Academic Press

ISBN: 9780080859095

Category: Science

Page: 394

View: 491

Yeast Cells

Yeast Gene Analysis

Finally, in certain cases it is desirable to identify the yeast counterpart of a
specific human gene. Subsequent characterization of the yeast ortholog using
the various genetic and recombinant technologies available in yeast permits
functional ...


Publisher: Academic Press

ISBN: 9780080860558

Category: Science

Page: 502

View: 685

Established almost 30 years ago, Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research. Focuses on the methods most useful for the functional analysis of yeast genes Allows researcher to identify which strategy to use without having to wade through numerous recipes Includes techniques for mutagenesis, transcript analysis, transposon tagging, and use of reporter genes Describes the use of tools for studying post-translational modifications in yeast Includes useful appendices with handy basic yeast recipes and WWW addresses

Yeast as a Tool in Cancer Research

STUDYING ANTICANCER DRUGS Jun O. Liu1 and Julian A. Simon2
1Departments of Pharmacology and Neuroscience, Johns Hopkins School of
Medicine, ...

Author: John L Nitiss

Publisher: Springer Science & Business Media

ISBN: 1402059639

Category: Medical

Page: 433

View: 183

Leland H. Hartwell Director, Fred Hutchinson Cancer Research Center, Nobel Laureate for Medicine, 2001 Yeast has proved to be the most useful single-celled organism for studying the fundamental aspects of cell biology. Resources are now available for yeast that greatly simplify and empower new investigations, like the presence of strains with each gene deleted, each protein tagged and databases on protein–protein interactions, gene regulation, and subcellular protein location. A powerful combination of genetics, cell biology, and biochemistry employed by thousands of yeast researchers has unraveled the complexities of numerous cellular processes from mitosis to secretion and even uncovered new insights into prion diseases and the role of prions in normal biology. These insights have proven, time and again, to foretell the roles of proteins and pathways in human cells. The collection of articles in this volume explores the use of yeast in pathway analysis and drug discovery. Yeast has, of course, supplied mankind’s most ubiquitous drug for thousands of years. In one aspect, the role of yeast in drug discovery is much like the role of yeast in other areas of biology. Yeast offers the power of genetics and a repetoire of resources available in no other organism. Using yeast in the study of drug targets and metabolism can help to make a science of what has been largely an empirical activity. A science of drug discovery would permit rigorous answers to important questions.

Guide to Yeast Genetics and Molecular and Cell Biology Part C

[ 1 ] Digital Time - Lapse Microscopy of Yeast Cell Growth By STEPHEN J. KRON
Introduction The vast number of yeast experiments are performed with
macroscopic colonies , which may contain 108 or more cells . The behavior of a
colony ...

Author: Christine Guthrie

Publisher: Gulf Professional Publishing

ISBN: 9780121822545

Category: Science

Page: 735

View: 109

This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.


10.1 General Aspects In the previous chapter, it was repeatedly pointed out what
consequences the loss of or mutations in essential yeast genes might have for
cell viability. As many of these genes participate in basic cellular functions that ...

Author: Horst Feldmann

Publisher: John Wiley & Sons

ISBN: 3527644865

Category: Science

Page: 348

View: 850

Yeast is one of the oldest domesticated organisms and has both industrial and domestic applications. In addition, it is very widely used as a eukaryotic model organism in biological research and has offered valuable knowledge of genetics and basic cellular processes. In fact, studies in yeast have offered insight in mechanisms underlying ageing and diseases such as Alzheimers, Parkinsons and cancer. Yeast is also widely used in the lab as a tool for many technologies such as two-hybrid analysis, high throughput protein purification and localization and gene expression profiling. The broad range of uses and applications of this organism undoubtedly shows that it is invalubale in research, technology and industry. Written by one of the world's experts in yeast, this book offers insight in yeast biology and its use in studying cellular mechanisms.

Brewing Yeast Fermentation Performance

Blomberg, A. (1997) The osmotic hypersensitivity of the yeast Saccharomyces
cerevisiae is strain and growth media dependent: quantitative aspects of the
phenomenon. Yeast 13, 529–539. Martin, V., Quain, D.E. and Smart, K.A. (1999)
The ...

Author: Katherine Smart

Publisher: John Wiley & Sons

ISBN: 0470695293

Category: Technology & Engineering

Page: 336

View: 652

Building on the success of the first edition, Brewing Yeast Fermentation Performance, Second edition considers the importance of yeast quality on fermentation performance and the means by which process control may therefore be achieved. Contributions from leading international brewing technologists from industry, research institutes and academia ensure that the coverage is practically oriented, commercially relevant and academically rigorous. Contents include up-to-date coverage of key aspects of the subject, including molecular innovations, yeast stress responses, wort composition, yeast quality, beer flavour development and yeast handling. Brewing Yeast Fermentation Performance is an essential purchase for commercial brewers at all levels, technical personnel and allied traders associated with the brewing industry. It is an excellent companion reference source to the first edition, covering complimentary topics that no one connected to the brewing industry can afford to be without. Libraries in universities and research establishments where food and beverage science and technology and microbiology are studied and taught should have multiple copies on their shelves.

The Yeast Nucleus

9 Yeast nuclear transport CHARLES N. COLE 1. Introduction The presence of a
nucleus is one of the defining characteristics of eukaryotic cells . Nuclei are
bounded by a double - membraned nuclear envelope . This separation of the ...

Author: Reader Institute of Cell and Molecular Biology Peter Fantes

Publisher: Oxford University Press, USA

ISBN: 9780199637720

Category: Science

Page: 316

View: 957

This book is concerned with the various nuclear activities of two yeasts: Saccharomyces cerevisiae and Schizosaccharomyces pombe. Both are excellent models for higher eukaryotes, including humans.

Yeast Biotechnology Diversity and Applications

T. Satyanarayana, Gotthard Kunze. Chapter 32 Nitrile Metabolizing Yeasts Tek
Chand Bhalla, Monica Sharma, and Nitya Nand Sharma Contents 32.1
Introduction . ... 727 32.3.4 Characteristics of Nitrile Metabolizing Activities of
Yeast .

Author: T. Satyanarayana

Publisher: Springer Science & Business Media

ISBN: 1402082924

Category: Science

Page: 744

View: 725

I belie ve that the book would provide an overview of the recent developments in the domain of yeast research with some new ideas, which could serve as an inspiration and challenge for researchers in this field. Ne w Delhi Prof. Asis Datta Dec. 24, 2007 F ormer Vice-chancellor, JNU Director, NCPGR (New Delhi) Pr eface Yeasts are eukaryotic unicellular microfungi that are widely distributed in the natural environments. Although yeasts are not as ubiquitous as bacteria in the na- ral environments, they have been isolated from terrestrial, aquatic and atmospheric environments. Yeast communities have been found in association with plants, a- mals and insects. Several species of yeasts have also been isolated from specialized or extreme environments like those with low water potential (e. g. high sugar/salt concentrations), low temperature (e. g. yeasts isolated from Antarctica), and low oxygen availability (e. g. intestinal tracts of animals). Around 1500 species of yeasts belonging to over 100 genera have been described so far. It is estimated that only 1% of the extant yeasts on earth have been described till date. Therefore, global efforts are underway to recover new yeast species from a variety of normal and extreme environments. Yeasts play an important role in food chains, and carbon, nitrogen and sulphur cycles. Yeasts can be genetically manipulated by hybridization, mutation, rare m- ing, cytoduction, spheroplast fusion, single chromosomal transfer and transfor- tion using recombinant technology. Yeasts (e. g.

Chinese Red Yeast Rice

For these reasons , some are advocating the use of red yeast in lieu of nitrates ,
which are commonly used to preserve foods like bacon , lunch meats , etc.
Nitrites are considered potential carcinogens . Safety Issues While adverse
effects have ...

Author: Rita Elkins

Publisher: Woodland Publishing

ISBN: 9781580540339

Category: Health & Fitness

Page: 32

View: 875

Mix the latest scientific data on herbal products and physical ailments with the need for natural approaches to health, and what do you have? The Woodland Health Series. Each of these booklets gives concise, pertinent information for those looking to nature for optimal health.

Galactose Regulon of Yeast

3.1 Genetic Analysis of GAL Regulon 3.1.1 Introduction How does yeast translate
the three-dimensional information of galactose into a specific biological signal
that activates transcription of GAL genes? Genetic analysis is the method of ...

Author: Paike Jayadeva Bhat

Publisher: Springer Science & Business Media

ISBN: 9783540740155

Category: Science

Page: 220

View: 529

This self contained book presents a comprehensive overview of the past, present and future of the galactose regulon of yeast, the classical model system of molecular biologists. The book starts with a brief historical overview on yeast research. This is followed by molecular genetics of the galactose regulon, isolation of genes and testing of the hypotheses. Contemporary topics including genomics, evolution, binary and graded responses, and stochasticity are all addressed.

Brewing Yeast and Fermentation

7.1.1 Maintenance of stock cultures The storage and assured supply of brewing
yeast strains is an important step in the propagation cycle. Although arguably
obvious, it is perhaps not generally appreciated that storage and supply are
critical ...

Author: Christopher Boulton

Publisher: John Wiley & Sons

ISBN: 1118685342

Category: Technology & Engineering

Page: 656

View: 175

Now Available for the First Time in Paperback! This unique volume provides a definitive overview of modern and traditional brewing fermentation. Written by two experts with unrivalled experience from years with a leading international brewer, coverage includes all aspects of brewing fermentation together with the biochemistry, physiology and genetics of brewers' yeast. Brewing Yeast and Fermentation is unique in that brewing fermentation and yeast biotechnology are covered in detail from a commercial perspective. Now available for the first time in paperback, the book is aimed at commercial brewers and their ingredient and equipment suppliers (including packaging manufacturers). It is also an essential reference source for students on brewing courses and workers in research and academic institutions. Definitive reference work and practical guide for the industry. Highly commercially relevant yet academically rigorous. Authors from industry leading brewers.

The Lost Art of Baking with Yeast Pastries

May this book bring into your household some of this happiness as well as the
memories, smells and tastes that I have grown up with. Baking with yeast is a gift
that I hope my Savta can impart to you. Baking with yeast is a lost art, and Savta
is ...

Author: Baba Schwartz


ISBN: 1458731359


Page: 132

View: 238

Baking with yeast is becoming a lost art. Many cooks would love to utilise the incredible properties of yeast, but lack a guide to inform and inspire them. The Lost Art of Baking with Yeast shows how simple baking with yeast can be, and how irresistible the results. The book includes recipes for cakes, slices, pastries, buns ... and Baba's famous golden dumpling cake. Baba Schwartz introduces the principles of yeast baking and gives handy hints for kneading and proving dough to perfection. These recipes, with their distinctive Hungarian flavour, will delight your family and friends. If you love baking, you will love discovering these recipes, some unique and some classic.

Gene Expression in Algae and Fungi Including Yeast

Language : English Descriptors : Saccharomyces ccrevisiac ; Mei . hioninc ;
Biosynthesis ; ( ienetic regulation ; Amino acid metabolism ; Gene expression
Abstract : The first step of the specific pathway for methionine biosynthesis in the
yeast ...

Author: Janet Saunders

Publisher: DIANE Publishing

ISBN: 9780788121678


Page: 59

View: 708

Includes 137 citations from the AGRICOLA database on the topic of gene expression in algae and fungi, including deuteromycotina, mold and yeast. Most citations have abstracts. Author and subject index.